Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5273038 | Tetrahedron Letters | 2009 | 4 Pages |
Abstract
Fluorescein isothiocyanate (FITC) is an amine reactive derivative of fluorescein dye that has wide ranging application in biochemistry. It has been extensively used to label peptides and proteins. However, its use in solid phase peptide synthesis is restricted. Indeed, in acidic conditions required for linker cleavage, N-ter FITC-labeled peptides undergo a cyclization leading to the formation of a fluorescein with subsequent removal of the last amino acid. This can be avoided when a spacer such as amino hexanoic acid is used or if non-acidic cleavage is operated to release targeted peptide from the resin.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Magali Jullian, Anaïs Hernandez, Amélie Maurras, Karine Puget, Muriel Amblard, Jean Martinez, Gilles Subra,