Article ID Journal Published Year Pages File Type
5273300 Tetrahedron Letters 2013 4 Pages PDF
Abstract

The synthesis of a compound containing a 4-nitrocatechol bound to two vicinal α-l-arabinofuranosyl moieties through a linker arm was achieved using a sulfate protecting group to facilitate selective alkylation of one aromatic hydroxyl. Several α-l-arabinofuranosidases displaying different selectivities were tested and a simple microtiter plate-based assay was developed. The observed resistance of the compound to α-l-arabinofuranosidase-mediated hydrolysis makes it suitable for the identification of enzymes that are able to accommodate bis-arabinofuranosylated moieties.

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Related Topics
Physical Sciences and Engineering Chemistry Organic Chemistry
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