Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5273300 | Tetrahedron Letters | 2013 | 4 Pages |
Abstract
The synthesis of a compound containing a 4-nitrocatechol bound to two vicinal α-l-arabinofuranosyl moieties through a linker arm was achieved using a sulfate protecting group to facilitate selective alkylation of one aromatic hydroxyl. Several α-l-arabinofuranosidases displaying different selectivities were tested and a simple microtiter plate-based assay was developed. The observed resistance of the compound to α-l-arabinofuranosidase-mediated hydrolysis makes it suitable for the identification of enzymes that are able to accommodate bis-arabinofuranosylated moieties.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Vinciane Borsenberger, Emmie Dornez, Marie-Laure Desrousseaux, Christophe M. Courtin, Michael J. O'Donohue, Régis Fauré,