A novel entry to 2′-O-aminopropyl modified nucleosides amenable for further modifications

2′-O-aminopropyl modified 4,6-difluoro-substituted benzimidazole nucleosides were synthesized in a two-step synthesis via Michael reaction and a Raney-nickel catalyzed reduction in high yields. These building blocks were used as a starting point for the conjugation of different carboxylic acids to enhance the lipophilicity or cationic character of oligonucleotides when used in biological assays or medical applications.

Graphical abstractA high yield sequence of Michael addition and Raney-Ni-reduction for 2′-RNA-modification.Download full-size image