Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5275380 | Tetrahedron Letters | 2012 | 5 Pages |
Abstract
Spliced leader (SL) RNA trans-splicing adds a N2,N2,7-trimethylguanosine cap (TMG) and a 22-nucleotide sequence, the SL, to the 5â² end of mRNAs. Both non-trans-spliced with a monomethylguanosine cap (MMG) and trans-spliced mRNAs co-exist in trans-splicing metazoan cells. Efficient translation of TMG-capped mRNAs in nematodes requires a defined core of nucleotides within the SL sequence. Here we present a chemical procedure for the preparation and purification of 5â²-terminal capped MMG and TMG wild-type, and mutant 22Â nt spliced leader RNAs (GGU/ACUUAAUUACCCAAGUUUGAG) with or without a 3â² biotin tag.
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Karolina Piecyk, Richard E. Davis, Marzena Jankowska-Anyszka,