Article ID Journal Published Year Pages File Type
5278725 Tetrahedron Letters 2009 4 Pages PDF
Abstract

[2′,3′,5′,6′-2H4]-2-Hydroxynaringenin is synthesised and incubated with commercially available UDP-glucose and the crude protein extract from Desmoduim uncinatum leaves. The organic extract produces isotopically labelled [2′,3′,5′,6′-2H4]-vitexin and [2′,3′,5′,6′-2H4]-isovitexin. Repeating the experiment with denatured protein or replacing the 2-hydroxynaringenin with [2′,3′,5′,6′-2H4]-apigenin or [2′,3′,5′,6′-2H4]-naringenin results in no observable incorporation. 2-Hydroxynaringenin is therefore the substrate for C-glucosylflavonoid biosynthesis in D. uncinatum.

Graphical abstract2-Hydroxynaringenin is C-glucosylated by UDP-glucose and a protein extract from Desmodium uncinatum leaves to afford vitexin and isovitexin. This is the first committed step to C-glucosylflavonoids, a biologically active class of natural compounds.Download full-size image

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Physical Sciences and Engineering Chemistry Organic Chemistry
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