Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5281428 | Tetrahedron Letters | 2007 | 4 Pages |
A simple method for the synthesis of carboxyl-free peptides on cellulose membranes was improved and adapted for fully automated SPOT synthesis. Using 1,1â²-carbonyl-di-imidazole (CDI) or 1,1â²-carbonyl-di-(1,2,4-triazole) (CDT) as an activator within a defined period of time, we were able to reduce the formation of di- or oligomerization of the C-terminal amino acid. The soluble peptides are obtained in a purity range of 60-95% and could be used directly for different biological assays (e.g., CD8 T-cell epitope) that require authentic C-termini.
Graphical abstractDirect coupling of CDI activated amino acids on cellulose membranes allows the synthesis of cleavable peptides with free C-termini in yields of at least 50 μg/cm2 which are enough for any biological assays.Download full-size image