| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5283675 | Tetrahedron Letters | 2007 | 4 Pages |
Abstract
DNA chips consisting of DNA oligonucleotide probes immobilized on the surface of solid supports are very powerful tools for rapid analysis of multiple samples. In this Letter we describe a new method for the efficient synthesis of DNA probes without their serious elimination by use of a new hydrophobic 16-hyroxydecanoic linker and a new non-aqueous reagent of MeNH2/THF for the deprotection of the base and phosphate protecting groups on CPG resins. The elimination of DNA probes in this new method could be suppressed more than 20-fold compared with the previous method using a hexaethylene glycol linker and concd NH4OH. Moreover, we carried out SNPs detection by use of our DNA-CPG conjugate to show the utility of our new linker and deprotection conditions.
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Authors
Akihiro Ohkubo, Haruhiko Taguchi, Kohji Seio, Hiroshi Nagasawa, Toshifumi Tsukahara, Mitsuo Sekine,