| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5284498 | Tetrahedron Letters | 2006 | 4 Pages |
Abstract
A methodology to synthesize oligonucleotides containing an alkyl interstrand cross-link between the two O6 atoms of deoxyguanosine has been developed. This cross-link is designed to serve as a stable structural mimic of the lesion formed in duplex DNA with the bifunctional alkylating agent hepsulfam. The O6-alkyl coupling is performed via a Mitsunobu reaction between a nucleoside and mono-protected 1,7-heptanediol. Solid-phase oligonucleotide synthesis using a nucleoside bis-phosphoramidite allows for the assembly of the cross-linked duplex. Sufficient quantities of this cross-linked duplex were obtained for various structural and biological investigations.
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Christopher J. Wilds, Jason D. Booth, Anne M. Noronha,