Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5348607 | Applied Surface Science | 2015 | 8 Pages |
Abstract
This work aimed to prepare a novel double-layer structure molecularly imprinted polymer film (MIF) on the surface plasmon resonance (SPR) sensor chips for detection of testosterone in aqueous media. The film was synthesized by in-situ UV photo polymerization. Firstly, the modification of gold surface of SPR chip was performed by 1-dodecanethiol. Then double-layer MIF was generated on the 1-dodecanethiol modified gold surface. The non-modified and imprinted surfaces were characterized by atomic force microscopy (AFM), fourier transform infrared (FTIR) spectroscopy and contact angle measurements. Analysis of SPR spectroscopy showed that the imprinted sensing film displayed good selectivity for testosterone compared to other analogues and the non-imprinted polymer film (NIF). Within the concentrations range of 1Â ÃÂ 10â12-1Â ÃÂ 10â8Â mol/L, the coupling angle changes of SPR were linear with the negative logarithm of testosterone concentrations (R2Â =Â 0.993). Based on a signal/noise ratio of three, the detection limit was estimated to be 10â12Â mol/L. Finally, the developed MIF was successfully applied to the seawater detection of testosterone. The results in the experiments suggested that a combination of SPR sensing with MIF was a promising alternative method for detection of testosterone in aqueous media.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Physical and Theoretical Chemistry
Authors
Yuan Tan, Lijing Jing, Yonghong Ding, Tianxin Wei,