Lotus-leaf-like structured chitosan-polyvinyl pyrrolidone films as an anti-adhesion barrier

For postsurgical anti-adhesion barrier applications, lotus-leaf-like structured chitosan-PVP films were prepared using a solution casting method with dodecyltrichloro-immobilized SiO2 nanoparticles. We evaluated whether the lotus-leaf-like structured chitosan-PVP films (l-chitosan-PVP) could be applied as postsurgical anti-adhesion barriers. A recovery test using a tensile strength testing machine and measurement of crystallinity using X-ray diffraction indicated that films with 75% PVP were the optimal composition of the chitosan-PVP films. Also, dodecyltrichloro-immobilized SiO2 nanoparticles were synthesized and sprayed on the film after pretreatment with the instant bio-glue. Analysis of cell adhesion, proliferation, and anti-thrombus efficiency were performed via a WST assay, field emission scanning electron microscopy, and hemacytometry. The contact angle with the lotus-leaf-like surface was of approximately 150°. Furthermore, the l-chitosan-PVP film yielded a lower cell and platelet adhesion rate (around less than 4%) than that yielded by the untreated film. These results indicate that the lotus-leaf-like structure has a unique property and that this novel l-chitosan-PVP film can be applied as a blood/tissue-compatible, biodegradable material for implantable medical devices that need an anti-adhesion barrier.