Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5350066 | Applied Surface Science | 2014 | 6 Pages |
Abstract
This work proposes a novel approach for protein purification from solution using mixed matrix membranes (MMMs) comprising of hydroxyapatite (HAP) inside polyether sulfone (PES) matrix. The influence of HAP particle loading on membrane morphology is studied. The MMMs are further characterized concerning permeability and adsorption capacity. The MMMs show purification of protein via both diffusion as well as adsorption, and show the potential of using MMMs for improvements in protein purification techniques. The bovine serum albumin (BSA) was used as a model protein. The properties and structures of MMMs prepared by immersion phase separation process were characterized by pure water flux, BSA adsorption and scanning electron microscopy (SEM).
Related Topics
Physical Sciences and Engineering
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Physical and Theoretical Chemistry
Authors
Junfen Sun, Lishun Wu,