| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5353088 | Applied Surface Science | 2016 | 33 Pages |
Abstract
Elastic properties of the substrate have profound effect on adhesion and proliferation of cells. Here, we introduce a method to produce polydimethylsiloxane (PDMS) substrates with stiffness tuned monotonically from 1.67 to 0.24Â MPa, by the time of UV irradiation adjusted up to 5Â h. The Young's modulus (determined by using nanoindenter) scales linearly with stiffness calculated using AFM-based force spectroscopy data. Such a relation enables the determination of the Young modulus from AFM force - distance curves also when the Herz model is not applicable. Our findings demonstrate that surface properties of PDMS substrates are not affected by the applied methodology of tuning substrate elasticity. Finally, the colorimetric proliferation assay (MTT) carried out for non-malignant (HCV29) and cancerous (T24) bladder cancer cells depicted a significant contribution of PDMS substrate elasticity to the behavior of cells. The softer PDMS substrate demonstrated excellent cytocompatibility whereas the stiff one is more cell-repellent.
Related Topics
Physical Sciences and Engineering
Chemistry
Physical and Theoretical Chemistry
Authors
Joanna Raczkowska, Szymon Prauzner-Bechcicki, Jaroslav Lukes, Josef Sepitka, Andrzej Bernasik, Kamil Awsiuk, CzesÅawa Paluszkiewicz, Joanna Pabijan, MaÅgorzata Lekka, Andrzej Budkowski,