Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5357070 | Applied Surface Science | 2014 | 5 Pages |
Abstract
This paper describes the development of a DNA sensor based on polypyrrole nanowire. By using potentiostatic technique, in the presence of gelatin as the soft mold, the polypyrrole nanowires were synthesized on the surface of the micro-sensor. The surface enhanced Raman spectroscopy shows that the NH ends of the polypyrrole nanowires orientate upward from the surface facilitating the DNA probe immobilization through the simple linkage with the phosphate groups of the probe DNA. The label-free signal readout was carried out by lock-in amplifier technique. The response time of the DNA sensor is 10Â s and the measurement time was 5Â min. The lowest detectable concentration of Escherichia coli DNA was 0.1Â nM.
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Authors
Mai Anh Tuan, Duc Thanh Pham, Thi Xuan Chu, Nguyen Minh Hieu, Nguyen Hoang Hai,