Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5358357 | Applied Surface Science | 2014 | 21 Pages |
Abstract
This paper reports an easy technique for immobilization of the DNA to the conducting polymer polypyrrole nanowires (PPy NWs). The nanowires were electrochemically synthesized on the surface of working electrode in the presence of gelatin as a soft mold. The structure of obtained PPy NWs was investigated by Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FTIR) spectroscopy and Surface Enhanced Raman Spectroscopy (SERS). The DNA strands were directly immobilized on the PPy NWs. The amino groups at the up-end of the PPy nanowires facilitate the linkage with the phosphate groups of the probe DNA. The DNA immobilization and hybridization were characterized by Electrochemical Impedance Spectroscopy (EIS). The initial results show that the sensor responses to 10Â pM of DNA sequence in the solution.
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Chemistry
Physical and Theoretical Chemistry
Authors
Thi Luyen Tran, Thi Xuan Chu, Dang Chinh Huynh, Duc Thanh Pham, Thi Hoai Thuong Luu, Anh Tuan Mai,