Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5361926 | Applied Surface Science | 2008 | 4 Pages |
Abstract
The effect of choline oxidase (Chox) on the electrochemical polymerization of poly(3,4-ethylenedioxythiophene) (PEDOT) was described. Result shows that the growth rate of PEDOT film varied with the addition of Chox quantity. The properties of PEDOT and PEDOT/Chox films were characterized by XPS and UV-vis absorption techniques. Meanwhile, a novel UV-vis absorbance method for the detection of choline was achieved. In a medium of 1/30Â M phosphate buffer solution (pH 8.0), a very sensitive absorbance peak at 293Â nm was observed. The linear relationship between UV-vis absorption intensity and choline concentration in a range from 1.0Â ÃÂ 10â6 to 5.1Â ÃÂ 10â5Â M was obtained. This method has also been applied to determine choline in several feedstuff additive samples.
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Authors
Hongjuan Zeng, Yadong Jiang, Junsheng Yu, Guangzhong Xie,