Study on the binding equilibrium between surfactant FC95 and DNA by resonance light-scattering technique

The binding equilibrium of potassium perfluorooctanesulfonate (FC95) to DNA was studied by UV spectra and resonance light scattering technique in the present paper. It was found that the interaction resulted in strongly enhanced resonance light scattering signals characterized by the RLS peak at 370 nm. The results indicated that the conformation of the DNA has changed during the interaction because the micro environment of DNA changed. The binding constant Kf and the number of binding n were calculated according to the equilibrium equation, which was established based on the data of the resonance light scattering measurements at 370 nm. It was found that the binding Kf and the number of binding n were 6.02 × 103 and 2.0, respectively.

Research highlights▶ FC95 was used to determine DNA. ▶ RLS technique is used in comprehension of the binding of PFOS to DNA. ▶ The mechanisms of the interaction was studied by UV spectra and RLS spectra.