Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5370528 | Applied Surface Science | 2005 | 7 Pages |
Abstract
Using two different 25-mer oligonucleotide probes covalently grafted on a silicon substrate, we demonstrate how efficient atomic force microscopy (AFM) can be for monitoring each step of DNA chip preparation: from probe immobilization to hybridization on the molecular scale. We observed the probe-molecule organization on the chip after immobilization, and the target molecules, which hybridized with probes could be individually identified. This article presents a method of straightforwardly identifying not only single and double DNA strands, but also, and more significantly, the hybridized part on them.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Physical and Theoretical Chemistry
Authors
Marie Hélène Rouillat, Vincent Dugas, Jean René Martin, Magali Phaner-Goutorbe,