Article ID Journal Published Year Pages File Type
5370528 Applied Surface Science 2005 7 Pages PDF
Abstract

Using two different 25-mer oligonucleotide probes covalently grafted on a silicon substrate, we demonstrate how efficient atomic force microscopy (AFM) can be for monitoring each step of DNA chip preparation: from probe immobilization to hybridization on the molecular scale. We observed the probe-molecule organization on the chip after immobilization, and the target molecules, which hybridized with probes could be individually identified. This article presents a method of straightforwardly identifying not only single and double DNA strands, but also, and more significantly, the hybridized part on them.

Related Topics
Physical Sciences and Engineering Chemistry Physical and Theoretical Chemistry
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