Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5371138 | Biophysical Chemistry | 2013 | 6 Pages |
â¢Modelling the interplay of Cdk1:CycB, Cdc20, APC/C, Emi2 and PP2A-Bâ²56 in the control of early embryonic cell cycleâ¢The period and amplitude of early embryonic cell cycle are sensitive to Emi2 levels.â¢Emi2 interferes with intrinsic time delay in APC/C activation and inactivation.
The early embryonic Xenopus cell cycles are characterized by alternating oscillations of Cyclin-dependent kinase-1 (Cdk1) and Anaphase Promoting Complex/Cyclosome (APC/C) activities. The early cycles before midblastula transition lack significant inhibitory Cdk1 phosphorylations and are driven by periodic accumulation of Cyclin B before M phase and its degradation by APC/C at the end of M phase. Both experiments and mathematical modelling suggest that while Cdk1:CycB phosphorylation activates APC/C, it inhibits its co-activator Cdc20 (Fizzy). These interactions create an amplified negative-feedback loop which is at the heart of all cell cycle oscillations. Recent experiments find that the APC/C inhibitor, Emi2/XErp1 is essential for large amplitude and short period Cyclin B oscillations during early divisions in the intact Xenopus embryo. This finding is counter-intuitive since larger amplitudes should come with slower cycle times. We explain this paradox by analysing the amplified negative feedback model extended with APC/C inhibition by Emi2. We show that Emi2 interferes with the intrinsic time-delay in APC/C activation and inactivation to increase the amplitude as well as shorten the period of Cyclin B oscillation.
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