| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5371394 | Biophysical Chemistry | 2011 | 8 Pages |
Abstract
⺠Urea-induced folding/unfolding monitored by far-UV circular dichroism and fluorescence spectroscopies. ⺠Equilibrium data for Ubq-UIM is inconsistent with a two-state model. ⺠Analysis of the kinetics of folding suggesting that formation of Ubq-domain is independent of UIM. ⺠A thermodynamic linkage model that involves four-states is applicable to the urea-induced unfolding of Ubq-UIM. ⺠The applicability of the model is further supported by variants of Ubq-UIM that have altered stabilities of domains.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Physical and Theoretical Chemistry
Authors
Mayank M. Patel, Franco Tzul, George I. Makhatadze,