| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5371528 | Biophysical Chemistry | 2011 | 7 Pages |
Abstract
⺠We showed that the simple molecular engineering of DNA aptamers sensitive to the thrombin based on hybridization of their supporting part resulted in formation of aptamers dimers that maintain the 3D conformation of the binding site - the guanine quadruplexes. ⺠The aptamer dimers had higher affinity to the thrombin as it has been demonstrated by their approximately two-fold stronger inhibitory effect on cleavage the fibrinogen by thrombin in comparison with those of canonical monomeric aptamers. ⺠We showed that the molecular engineering can be used as effective tool for improvement of the aptamer properties.
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Authors
SlavomÃra Poniková, KatarÃna TluÄková, Marián AntalÃk, Viktor VÃglaský, Tibor Hianik,