Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5371627 | Biophysical Chemistry | 2010 | 5 Pages |
Abstract
Lysenin forms unitary large conductance pores in artificial bilayer membranes containing sphingomyelin. A population of lysenin pores inserted into such a bilayer membrane exhibited a dynamic negative conductance region, as predicted by a simple two-state model for voltage-gated channels. The recorded I-V curves demonstrated that lysenin pores inserted into the bilayer are uniformly oriented. Additionally, the transition between the two-states was affected by changes in the monovalent ion concentration and pH, pointing towards an electrostatic interaction governing the gating mechanism.
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Physical Sciences and Engineering
Chemistry
Physical and Theoretical Chemistry
Authors
Daniel Fologea, Eric Krueger, Rachel Lee, Matt Naglak, Yuriy Mazur, Ralph Henry, Greg Salamo,