Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5371707 | Biophysical Chemistry | 2010 | 6 Pages |
Abstract
We have proposed the formation of Schiff-base between R96 and chromophore (CRO) to elucidate the reaction mechanism for the irreversible red shift of green fluorescent protein (GFP) spectra under the absence of oxygen. The difference between absorption energies of reactant and product for our GFP models with CIS(D)/6-31G* level is 0.21Â eV, which is in reasonable agreement with the corresponding experimental value of 0.25Â eV. We have suggested the irreversible photoreaction mechanism, where the CRO excited from ground (S0) state to first excited singlet (S1) state immediately turns to the first excited triplet (T1) state, and the nucleophilic addition reaction occurs on the T1 state.
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Authors
Jun Koseki, Yukiumi Kita, Masanori Tachikawa,