Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5372482 | Biophysical Chemistry | 2006 | 5 Pages |
Abstract
Herein a new method that allows binding ÎCp to be determined with a single experiment is presented. Multithermal titration calorimetry (MTC) is a simple extension of isothermal titration calorimetry (ITC) that explicitly takes into account the thermal dependences of ÎH and the binding constant. Experimentally, this is accomplished by performing a single stepwise titration with ITC equipment, allowing temperature re-adjustments of the system at intermediate states of the titration process. Thus, from the resulting multitherm, ÎCp can also be determined. The experimental feasibility of MTC was tested by using the well-characterized lysozyme-chitotriose complex as a model system.
Related Topics
Physical Sciences and Engineering
Chemistry
Physical and Theoretical Chemistry
Authors
Eneas A. Chavelas, Rafael A. Zubillaga, Nancy O. Pulido, Enrique GarcÃa-Hernández,