Article ID Journal Published Year Pages File Type
5374726 Chemical Physics 2011 14 Pages PDF
Abstract
► Photo-activated adenylyl cyclase (nPAC) from Naegleria gruberi NEG-M was expressed. ► Photodynamics of BLUF domain in BLUF sensor - cyclase actuator protein was studied. ► Photo-excitation caused BLUF photo-cycling and permanent protein re-conformation. ► Re-conformed protein enabled photo-induced flavin reduction by proton transfer. ► Fluorescence of flavin in dark- and light-adapted state of nPAC was characterized.
Related Topics
Physical Sciences and Engineering Chemistry Physical and Theoretical Chemistry
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