Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5375485 | Chemical Physics | 2009 | 8 Pages |
Abstract
The fluorescence spectroscopic behaviour of (6R,S)-5,10-methenyltetrahydrofolate (MTHF), (6R,S)-10-formyltetrahydrofolate (10-HCO-H4folate), 10-formyldihydrofolate (10-HCO-H2folate), and 10-formylfolate (10-HCO-folate) in aqueous Tris-HCl buffer at pH 8 is studied. MTHF and 10-HCO-folate were commercially available. 10-HCO-H4folate was prepared from MTHF by hydrolysis at room temperature under anaerobic conditions. 10-HCO-H2folate was prepared by oxidation of 10-HCO-H4folate under aerobic conditions. Fluorescence quantum distributions at room temperature and fluorescence signal decays at room temperature and liquid nitrogen temperature were measured. The fluorescence lifetimes determined at room temperature (liquid nitrogen temperature) are 10Â ps (2.9Â ns) for MTHF, 38Â ps (3.7Â ns) for 10-HCO-H4folate, 80Â ps (10.5Â ns) for 10-HCO-H2folate, and 7.1Â ns (20Â ns) for 10-HCO-folate. The results are discussed in terms of dyadic (pterin-benzoyl-glutamate) photo-induced electron transfer and dyadic fluorescent dynamics.
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Authors
A. Tyagi, A. Penzkofer, A. Batschauer, E. Wolf,