| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5390873 | Chemical Physics Letters | 2006 | 7 Pages |
Abstract
Effect of lipid hydration on the structure of lamellar lipoplexes made of the cationic lipid 3-[N-(N,N-dimethylaminoethane)-carbamoyl]cholesterol (DC-Chol), the neutral 'helper' lipid dioleoylphosphatidylethanolamine (DOPE) and calf-thymus DNA was investigated by synchrotron small angle X-ray diffraction (SAXD). Here, we show that lipid hydration is the key factor regulating the equilibrium structure of lipoplexes. Thermotropic behavior was also investigated between 5 and 65 °C. Both the membrane thickness and the water layer thickness were found to decrease linearly as a function of temperature while the one dimensional DNA rod lattice between lipid bilayers was found to enlarge. Structural results were interpreted in terms of recently proposed theoretical models.
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Authors
Daniela Pozzi, Heinz Amenitsch, Ruggero Caminiti, Giulio Caracciolo,