| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5391363 | Chemical Physics Letters | 2008 | 4 Pages |
Abstract
The paper studies the excitation intensity dependence of diffusion measurements of the fluorescent dye Cy5 using conventional fluorescence correlation spectroscopy (FCS) and dual-focus fluorescence correlation spectroscopy (2fFCS). Due to the light-driven transitions of Cy5 between the fluorescent and non-fluorescent states, conventional FCS shows a strong dependency of the apparent diffusion coefficient on excitation intensity, whereas 2fFCS is virtually free from that artefact. Moreover, we provide an absolute value for the diffusion coefficient of Cy5.
Related Topics
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Authors
Anastasia Loman, Thomas Dertinger, Felix Koberling, Jörg Enderlein,