Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5398413 | Journal of Luminescence | 2016 | 9 Pages |
Abstract
Water-soluble LaF3:Ce,Tb luminescent nanocrystals (RLNCs) were successfully fabricated according to previously reported literatures. The experimental results indicate that the as-prepared nanocrystals consist of well crystallized hexagonal phases, having a nearly spherical shape with an average diameter of 10Â nm. The interaction of RLNCs with bovine serum albumin (BSA) was studied mainly via fluorescence quenching in combination with circular dichroism (CD) and ultraviolet-visible (UV-vis) absorption spectroscopy under imitated physiological conditions. The fluorescence titration results reveal that RLNCs could efficiently quench the intrinsic fluorescence of BSA mainly through a dynamic quenching procedure. The binding constant and the number of binding site at 300Â K were estimated to be 4.606Ã103Â LÂ molâ1 and 0.98, respectively. Meanwhile, the thermodynamic parameters for RLNCs-BSA system were also determined, suggesting that the binding reaction between RLNCs and BSA took place spontaneously and was primarily driven by hydrophobic forces. Furthermore, it was found that the binding of RLNCs to BSA was mainly located in site I and the binding distance was estimated to be 3.0Â nm. Finally, the synchronous fluorescence, three dimensional (3D) fluorescence, and CD spectroscopy were used to explore the conformational alterations of protein induced by RLNCs.
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Authors
Xingjia Guo, Aijun Hao, Qiong Wu, Xin Diao, Wenjing Liu, Chenri Cong, Ye Sun, Liping Xu, Jie Yao,