Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5400329 | Journal of Luminescence | 2014 | 8 Pages |
Abstract
The primary objective of this study is to evaluate the interactions of human serum albumin (HSA) with peimine (PE) and peiminine (PEN) in physiological conditions by fluorescence spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, Raman spectroscopy, and molecular modeling. PE and PEN were isolated from Bulbus Fritillariae thunbergii miq. The binding constants Ka and the number of binding sites n were calculated at different temperatures. Enthalpy change (ÎH), entropy change (ÎS), and Gibbs free energy change (ÎG) were also determined. The results suggested that quenching of HSA fluorescence by PE and PEN is a static process. Three-dimensional fluorescence, FT-IR, CD, and Raman spectra showed that the binding of PE and PEN to HSA can induce conformational changes in the latter. Moreover, important differences in binding ability were observed between PE and PEN, and PE showed stronger binding affinity to HSA than PEN.
Related Topics
Physical Sciences and Engineering
Chemistry
Physical and Theoretical Chemistry
Authors
Dan Xiao, Lili Zhang, Qing Wang, Xia Lin, Jinyu Sun, Hui Li,