Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5400641 | Journal of Luminescence | 2013 | 7 Pages |
Abstract
Conjugates of gold nanoparticles (AuNPs) and semiconductor quantum dots (CdS/T) have been synthesized with bovine serum albumin (BSA) using wet chemistry. The optical properties of nano and quantum materials and their BSA conjugate have been studied using UV-Visible and Fluorescence spectroscopy. UV-Visible spectrum of pure BSA showed an absorption maximum at 278Â nm, which showed blue shift after its conjugation with nano and quantum materials. Increased concentration of AuNPs during conjugation resulted in broadening of BSA peak (278Â nm), which can be related to the formation of ground state complex formation, caused by the partial adsorption of BSA on the surface of NPs. However, increased concentrations of BSA resulted in decrease in SPR intensity of gold nanoparticles (528Â nm) and absorbance peak of BSA started diminishing. AuNPs acted as quencher for BSA fluorescence intensity, when excited at 280Â nm. The binding constant (K) and the number of binding sites (n) between AuNPs and BSA have been found to be 1.97Ã102 LMâ1 and 0.6 respectively. With quantum dots, conjugation resulted in enhancement of fluorescence emission of quantum dots when excited at 300Â nm, which might be due to the stabilizing effect of BSA on QDs or due to energy transfer from tryptophan moieties of albumin to quantum dots.
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Authors
Suman Singh, Rajnish Kaur, Jitender Chahal, P. Devi, D.V.S. Jain, M.L. Singla,