Probing the interaction of human serum albumin with vitamin B2 (riboflavin) and l-Arginine (l-Arg) using multi-spectroscopic, molecular modeling and zeta potential techniques

► We studied the interaction of riboflavin with HSA in presence and absence of l-Arg. ► Molecular modeling and zeta-potential used to describe competitive interaction. ► We compared the binding mechanism of riboflavin (RB) to HSA in both systems. ► We determined critical aggregation concentration of RB on HSA in both systems. ► The binding site of RB on HSA in both systems has been determined.