| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5402381 | Journal of Luminescence | 2010 | 5 Pages |
Abstract
The lifetime variations of enhanced cyan fluorescence protein (ECFP) in relatively short integration time bins were studied via time-correlated single photon counting (TCSPC) measurement. We observed that minimum photon counts are necessary for the lifetime estimation to achieve a certain range of variance. The conditions to decrease the variance of lifetime were investigated and the channel width of the measurement of TCSPC data was found to be another important factor for the variance of lifetime. Though the lifetime of ECFP is best fit by a double exponential, a mono exponential fit for the same integration time is more stable. The results may be useful in the analysis of photophysical dynamics for ensemble molecules in short measurement time windows.
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Authors
Soonhyouk Lee, Soo Yong Kim, Kyoungsook Park, Jinyoung Jeong, Bong Hyun Chung, Sok Won Kim,