Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5402578 | Journal of Luminescence | 2010 | 6 Pages |
Abstract
One-step aqueous synthesis of CdS nanoparticles as a novel fluorescence probe for sensitive and selective determination of DNA with synchronous fluorescence spectrometric method has been developed. Different from the traditional organometallic route, in which toxic precursors or solvents might be used, the wet chemical approach demonstrated in this paper is superior in terms of simplicity, using of nontoxic materials, mild synthetic condition and good reproducibility. When Îλ=255 nm, maximum synchronous fluorescence is produced at 264 nm, the synchronous fluorescence intensity of the composite nanoparticles is significantly decreased in the presence of trace DNA at PH 0.91. Under optimal conditions, the linear ranges of the calibration curves are 0.08-30.0 μg mLâ1 for ctDNA and 0.05-35.0 μg mLâ1 for hsDNA, respectively. The detection limits are 1.5 ng mLâ1 for ctDNA and 2.2 ng mLâ1 for hsDNA, respectively. Furthermore, the method is successfully applied to the quantification of DNA in synthetic samples. The results show that this proposed method is stable, sensitive and practical for the determination of trace DNA.
Related Topics
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Authors
Lun Wang, Jie Fu, Hongqi Chen, Ani Liang, Binbin Qian, Bo Ling, Cailing Zhou,