Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5402858 | Journal of Luminescence | 2010 | 6 Pages |
Abstract
We demonstrate that DiI and rhodamine B, which are not easily distinguishable to one-photon measurements, can be differentiated and in fact quantified in mixture via tailored two-photon excitation pulses found by a genetic algorithm (GA). A nearly three-fold difference in the ratio of two-photon fluorescence of the two dyes is achieved, without a drop in signal of the favored fluorophore. Implementing an acousto-optic interferometer, we were able to prove that the mechanism of discrimination is second-harmonic tuning by the phase-shaped pulses to the relative maxima and minima of these cross-sections.
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Authors
Eric R. Tkaczyk, Alan H. Tkaczyk, Koit Mauring, Jing Yong Ye, James R. Jr., Theodore B. Norris,