Fluorescence resonance energy transfer between acridine orange and rhodamine 6G and analytical application in micelles of dodecyl benzene sodium sulfonate

A new method for the determination of erythromycin by energy transfer fluorescence quenching of acridine orange (AO)-rhodamine 6G (R6G) in micelles solution studied and established. It was found that the effective energy transfer could occur between AO and R6G in the dodecyl benzene sodium sulfonate at λex/λem=470/553nm and Britton-Robinson buffer (BR, pH=5.72). The fluorescence intensity of R6G had been increased sharply. Erythromycin diminished the fluorescence intensity of R6G. The detection limit was up to 0.316 mg l−1. The range of determining concentration of erythromycin was 0.75-15 mg l−1. The relative standards deviation were 0.66-1.38% for six parallel determinations of erythromycin. The recoveries of erythromycin were 96.95-101.41%. The measurement was applied to the determination of capsules of erythromycin with good results.