Article ID Journal Published Year Pages File Type
5404400 Journal of Luminescence 2006 6 Pages PDF
Abstract
A new spectrofluorimetric method was developed for determination of trace amount of adenosine disodium triphosphate (ATP). Using norfloxacin (NFLX)-terbium (Tb3+) as a fluorescent probe, in the buffer solution of pH=7.40, ATP can remarkably enhance the fluorescence intensity of the NFLX-Tb3+ complex at λ=545nm and the enhanced fluorescence intensity of Tb3+ ion is in proportion to the concentration of ATP. Optimum conditions for the determination of ATP were also investigated. The dynamic range for the determination of ATP is 1.00×10−6-1.60×10−5 mol/L with detection limit of 4.13×10−8 mol/L. This method is simple, practical and relatively free interference from coexisting substances and can be successfully applied to determination of ATP in samples.
Related Topics
Physical Sciences and Engineering Chemistry Physical and Theoretical Chemistry
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