Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5404400 | Journal of Luminescence | 2006 | 6 Pages |
Abstract
A new spectrofluorimetric method was developed for determination of trace amount of adenosine disodium triphosphate (ATP). Using norfloxacin (NFLX)-terbium (Tb3+) as a fluorescent probe, in the buffer solution of pH=7.40, ATP can remarkably enhance the fluorescence intensity of the NFLX-Tb3+ complex at λ=545nm and the enhanced fluorescence intensity of Tb3+ ion is in proportion to the concentration of ATP. Optimum conditions for the determination of ATP were also investigated. The dynamic range for the determination of ATP is 1.00Ã10â6-1.60Ã10â5 mol/L with detection limit of 4.13Ã10â8 mol/L. This method is simple, practical and relatively free interference from coexisting substances and can be successfully applied to determination of ATP in samples.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Physical and Theoretical Chemistry
Authors
Yanhong Miao, Jinkai Liu, Faju Hou, Chongqiu Jiang,