A straightforward method for stereospecific assignment of val and leu prochiral methyl groups by solid-state NMR: Scrambling in the [2-13C]Glucose labeling scheme

▸ Stereospecific assignments can improve the accuracy and precision of NMR structures. ▸ New strategy for solid-state NMR stereospecific resonance assignment of Val and Leu. ▸ Based on protein production in Escherichia coli media containing [2-13C]Glucose. ▸ Spectral simplification in the often congested methyl region. ▸ Applications to PrgI needles and ubiquitin are shown.