Organ specific mapping of in vivo redox state in control and cigarette smoke-exposed mice using EPR/NMR co-imaging

► EPR/NMR co-imaging detected the distribution of a nitroxide probe in living mice. ► 3D mapping of the in vivo distribution and metabolism of the probe was performed. ► Organ specific differences of in probe uptake, metabolism and clearance were imaged. ► Reductive metabolism was markedly decreased in smoking-exposed mice. ► EPR/NMR co-imaging enabled imaging and mapping of this smoking-induced redox stress.