| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5406600 | Journal of Magnetic Resonance | 2010 | 7 Pages |
Abstract
By incorporating homonuclear decoupling on both the 1H and 13C channels it is feasible to obtain high-resolution two-dimensional separated local field spectra of peptides and proteins that are 100% labeled with 13C. Dual-PISEMO (Polarization Inversion Spin Exchange Modulated Observation) can be performed as a conventional two-dimensional experiment, or with windowed detection as a one-dimensional experiment that offers flexibility as a building block for shiftless and other multidimensional triple-resonance experiments with the inclusion of 15N irradiation. The triple-resonance MAGC probe used to perform these experiments at 500Â MHz is described.
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Authors
Eugene C. Lin, Chin H. Wu, Yuan Yang, Christopher V. Grant, Stanley J. Opella,