Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5406868 | Journal of Magnetic Resonance | 2009 | 7 Pages |
Abstract
A strip-shield inserted between a high inductance double-tuned solenoid coil and the glass tube containing the sample improves the efficiency of probes used for high-field solid-state NMR experiments on lossy aqueous samples of proteins and other biopolymers. A strip-shield is a coil liner consisting of thin copper strips layered on a PTFE (polytetrafluoroethylene) insulator. With lossy samples, the shift in tuning frequency is smaller, the reduction in Q, and RF-induced heating are all significantly reduced when the strip-shield is present. The performance of 800Â MHz 1H/15N and 1H/13C double-resonance probes is demonstrated on aqueous samples of membrane proteins in phospholipid bilayers.
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Authors
Chin H. Wu, Christopher V. Grant, Gabriel A. Cook, Sang Ho Park, Stanley J. Opella,