Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5407024 | Journal of Magnetic Resonance | 2009 | 9 Pages |
Abstract
As a result, optimal 13C spectra of the oxidized, paramagnetic form of human copper zinc superoxide dismutase (SOD) can be obtained employing rf-fields which do not exceed 40Â kHz during the whole experiment. This enables the removal of unwanted heating which can lead to deterioration of the sample. Furthermore, combined with the short 1H T1s, this allows the repetition rate of the experiments to be shortened from 3Â s to 500Â ms, thus compensating for the sensitivity loss due to the smaller sample volume in a 1.3Â mm rotor. The result is that 2D 13C-13C correlation could be acquired in about 24Â h on less than 1Â mg of SOD sample.
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Authors
Ségolène Laage, Joseph R. Sachleben, Stefan Steuernagel, Roberta Pierattelli, Guido Pintacuda, Lyndon Emsley,