Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5407274 | Journal of Magnetic Resonance | 2008 | 17 Pages |
Abstract
Inter-spin distances between 1Â nm and 4.5Â nm are measured by continuous wave (CW) and pulsed electron paramagnetic resonance (EPR) methods for a series of nitroxide-spin-labelled peptides. The upper distance limit for measuring dipolar coupling by the broadening of the CW spectrum and the lower distance limit for the present optimally-adjusted double electron electron resonance (DEER) set-up are determined and found to be both around 1.6-1.9Â nm. The methods for determining distances and corresponding distributions from CW spectral line broadening are reviewed and further developed. Also, the work shows that a correction factor is required for the analysis of inter-spin distances below approximately 2Â nm for DEER measurements and this is calculated using the density matrix formalism.
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Authors
J.E. Banham, C.M. Baker, S. Ceola, I.J. Day, G.H. Grant, E.J.J. Groenen, C.T. Rodgers, G. Jeschke, C.R. Timmel,