Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5407308 | Journal of Magnetic Resonance | 2007 | 12 Pages |
Abstract
In this publication, we introduce a version of the rotational resonance width experiment with a homogeneously broadened matching condition. The increase in the bandwidth is achieved by the reduction of the proton decoupling power during mixing, which results in the reduction of zero-quantum relaxation, and broadens the rotational resonance condition. We show that one can achieve recoupling of the carbonyl-aliphatic side chain dipolar interactions band selectively, while avoiding the recoupling of strongly interacting Câ²-Cα and Câ²-Cβ spin pairs. The attenuation of the multi-spin effects in the presence of short zero-quantum relaxation enables a two-spin approximation to be employed for the analysis of the experimental data. The systematic error introduced by this approximation is estimated by comparing the results with a three-spin simulation. The experiment is demonstrated in [U-13C,15N]N-acetyl-l-Val-l-Leu dipeptide, where 11 distances, ranging from 2.5 to 6 Ã
, were measured.
Related Topics
Physical Sciences and Engineering
Chemistry
Physical and Theoretical Chemistry
Authors
Rafal Janik, Xiaohu Peng, Vladimir Ladizhansky,