| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5407547 | Journal of Magnetic Resonance | 2006 | 7 Pages |
Abstract
The homonuclear scalar coupling of a directly bonded 13C-13C pair has been used to create a double-quantum filter (DQF) to remove the natural-abundance 13C background in 13C{15N} rotational-echo double-resonance (REDOR) experiments. The DQF scalar and REDOR dipolar evolution periods are coincident which is important for sensitivity in the event of weak 13C-15N dipolar coupling. Calculated and observed 13C{15N} DQF-REDOR dephasings were in agreement for a test sample of mixed recrystallized labeled alanines. Glycine metabolism in a single uniform-15N soybean leaf labeled for 6Â min by 13CO2 was measured quantitatively by 13C{15N} DQF-REDOR with no background interferences.
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Authors
Shigeru Matsuoka, Jacob Schaefer,