Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5407655 | Journal of Magnetic Resonance | 2007 | 8 Pages |
Abstract
By using the mixed solvent of 50% H2O/50% D2O and employing deuterium decoupling, TROSY experiments exclusively detect NMR signals from semideuterated isotopomers of carboxamide groups with high sensitivities for proteins with molecular weights up to 80Â kDa. This isotopomer-selective strategy extends TROSY experiments from exclusively detecting backbone to both backbone and side-chain amides, particularly in large proteins. Because of differences in both TROSY effect and dynamics between 15N-HE{DZ} and 15N-HZ{DE} isotopomers of the same carboxamide, the 15N transverse magnetization of the latter relaxes significantly faster than that of the former, which provides a direct and reliable stereospecific distinction between the two configurations. The TROSY effects on the 15N-HE{DZ} isotopomers of side-chain amides are as significant as on backbone amides.
Related Topics
Physical Sciences and Engineering
Chemistry
Physical and Theoretical Chemistry
Authors
Aizhuo Liu, Lishan Yao, Yue Li, Honggao Yan,