Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5407893 | Journal of Magnetic Resonance | 2006 | 14 Pages |
Abstract
REDOR is a solid-state NMR technique frequently applied to biological structure problems. Through incorporation of phosphorothioate groups in the nucleic acid backbone and mono-fluorinated nucleotides, 31P{19F} REDOR has been used to study the binding of DNA to drugs and RNA to proteins through the detection of internuclear distances as large as 13-14Â Ã
. In this work, 31P{19F} REDOR is further refined for use in nucleic acids by the combined use of selective placement of phosphorothioate groups and the introduction of nucleotides containing trifluoromethyl (-CF3) groups. To ascertain the REDOR-detectable distance limit between an unique phosphorous spin and a trifluoromethyl group and to assess interference from intermolecular couplings, a series of model compounds and DNA dodecamers were synthesized each containing a unique phosphorous label and trifluoromethyl group or a single 19F nucleus. The dipolar coupling constants of the various 31P and 19F or -CF3 containing compounds were compared using experimental and theoretical dephasing curves involving several models for intermolecular interactions.
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Physical Sciences and Engineering
Chemistry
Physical and Theoretical Chemistry
Authors
Elizabeth A. Louie, Panadda Chirakul, Vinodhkumar Raghunathan, Snorri Th. Sigurdsson, Gary P. Drobny,