Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5408029 | Journal of Molecular Liquids | 2017 | 11 Pages |
Abstract
Ion-pair complex of the chlorpheniramine maleate with eosin Y was achieved because of the interaction of protonated drug with dianionic eosin Y. The stoichiometry of the reaction was studied by mole ratio method which showed 2:1 ratio for drug and eosin Y. This lead to the quenching of eosin Y which was the basis for the quantitation of chlorpheniramine maleate by fluorescence spectrophotometry. The fluorescence intensity was recorded at 544.02 nm after excitation at 257.96 nm. The linear dynamic range was obtained in the concentration range of 1-8 μg mLâ 1. The quenching rate constant was calculated and found to be 8.2 Ã 1013 L molâ 1 sâ 1 which is more than 2 Ã 1010 L molâ 1 sâ 1 indicated that the proposed method has followed static quenching. The enthalpy change and Gibb's free energy were calculated and found to be negative. The same results were also obtained by computational calculations using Gaussian. Various variables such as reaction time, temperature, buffer solution and solvent were optimized and the proposed method was successfully applied for the assay of active chlorpheniramine maleate in commercial tablets.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Physical and Theoretical Chemistry
Authors
Syed Najmul Hejaz Azmi, Shamsa Said Khalfan Al-Hadhrami, Bashair Mohammed Rashid Al-Marhoubi, Safa Saleh Said Al-Sulaimi, Zainab Darwish Said Al-Shamoosi,