Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5409001 | Journal of Molecular Liquids | 2017 | 68 Pages |
Abstract
Bovine serum albumin acts as storage and a transport of endogenous and exogenous compounds with protein in the blood. Eletriptan hydrochloride is an anti-migraine headache drug and binding of this drug to albumin is necessary in pharmacokinetic and pharmacodynamics. Many metal ions are present in blood plasma, therefore, the study on the effects of metal ions on the interaction between drugs and plasma proteins are essential. In this study, the effects of metal ions Zn2 +, Fe2 + and Cu2 + on the interaction between bovine serum albumin (BSA) and eletriptan hydrochloride (ELT) were investigated by employing emission fluorescence, synchronous fluorescence, ultraviolet-visible (UV-Vis) absorption, Fourier transform infrared (FT-IR) spectroscopic and molecular docking studies at physiological buffer conditions (pH 7.4). Results show that static quenching, spontaneous process, hydrogen bonds and vander Waals interactions and the values of Ka, n , Kb, R0 , r and thermodynamic parameters were obtained at different temperatures; then the binding sites for ELT in BSA present in sub-domains IIA, IIIA and IB. The entire outcome indicates that the metal ions decrease the binding efficacy of ELT on BSA. The study of binding phenomena is important in providing basic information on the pharmacological actions, biotransformation and bio-distribution, and this would be useful in pharmaceutical industry, life sciences and clinical medicine.
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Authors
Manjushree M, Revanasiddappa Hosakere D.,