Investigation of inclusion complex of HP-γ-cyclodextrin with ceftazidime

The inclusion complex of ceftazidime (CF) with hydroxypropyl-γ-cyclodextrin (HP-γ-CD) was prepared. The inclusion behavior and interactions of CF with HP-γ-CD was carried out in both the solution and the solid state by UV/vis, FT-IR, NMR and molecular docking techniques. All the obtained information demonstrated the formation of CF-CD inclusion complex. The CF-CD inclusion complex exhibited different spectroscopic features and properties from CF. The results suggested that CF-CD inclusion complex was established with 1:1 stoichiometry. It was found that a part of CF molecule, the thiazole ring with sulfur and nitrogen atoms with CO and CN bands of CF was included in the HP-γ-CD cavity. Also the solubility and stability of CF-HP-γ-CD was significantly improved and consequently the bioavailability of CF through CF-CD inclusion complex was effectively improved over free CF. Based on the enhancement of absorbance of CF in the presence of HP-γ-CD a sensitive spectroscopic method for the CF determination was developed.