Stoichiometry and equilibrium constant of the complex of PAMAM-NH2 G4 and 5-fluorouracil

► PAMAM-NH2 G4 molecule has two sets of active sites that bind 5-fluorouracil (FU) ► Stoichiometry and equilibrium constants of the G4-FU complex were determined by the technique of equilibrium dialysis ► Dendrimer molecule has about 12 active sites that stronger bind FU (K1 = 5600) at 20 °C ► Moreover this dendrimer molecule has about 37 active sites that bind FU with lower equilibrium constant (K2 = 150) at 20 °C